Hart Biologicals Collagen Reagent is used to diagnose platelet dysfunction, or normal platelet activity in human platelet rich plasma or whole blood.
The platelet aggregation test measures the rate and degree to which dispersed platelets in a sample of platelet rich plasma (PRP) or anti-coagulated whole blood forms clumps (aggregates) after the addition of a substance that normally stimulates platelet aggregation (agonist).
Collagen is a structural protein which is virtually ubiquitous throughout the human body. During primary haemostasis following blood vessel injury, the adhesion of platelets to exposed collagen at the site of injury plays a key role in the arrest of bleeding from this site. Any interference with the ability of platelets to interact with exposed collagen is therefore a possible cause of an unexplained bleeding tendency. Some congenital defects of platelet function, as well as some acquired and drug-induced effects can affect the aggregation of platelets stimulated by collagen. Investigation of the ability of patient platelets to aggregate to a controlled preparation of collagen fibrils is an important part of the investigation of suspected disorder of platelet function
Hart Biologicals Collagen Reagent is supplied as the following for reconstitution with 1.0ml distilled water:
· 1 x 1.0ml 100ug/ml Collagen